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Objective:To analyze the application value of XpertMTB/RIF, culture of Mycobacterium tuberculosis (MTB), and detection of Mycobacterium tuberculosis DNA (TB-DNA) in the diagnosis of tuberculosis.Methods:From January 2018 to January 2020, 75 patients admitted to Wenzhou Central Hospital and diagnosed with tuberculosis in clinic were selected in the research.The results of clinical diagnosis were used as the gold standard.The sensitivity, specificity and accuracy of XpertMTB/RIF, TB cultureand TB-DNA in the detection of tuberculosis were analyzed.The resistance of MTB to rifampicin and the adverse reactions such as blood in sputum and fever of patients were evaluated.Results:The sensitivity, specificity and accuracy of XpertMTB/RIF in detecting tuberculosis were 95.24%, 72.71%and 85.33%, respectively.The sensitivity, specificityand accuracy of TB culture in detecting tuberculosis were 80.95%, 66.67% and 74.67%, respectively.The sensitivity, specificity and accuracy of TB-DNA detection in detecting tuberculosis were 78.57%, 69.70%and 74.67%, respectively.There were statistically significant differences in the sensitivity among XpertMTB/RIF, TBand TB-DNA in detecting tuberculosis (χ 2=4.086, 5.126, P=0.043, 0.024). The result of clinical diagnosis was used as the gold standard.In the positive samples obtained, the sensitivity and specificity of XpertMTB/RIF in detection of drug resistance were 72.73% and 93.55%, respectively.The sensitivity and specificity of TB were 54.55% and 74.19%, respectively, and the sensitivity and specificity of TB-DNA were 63.64% and 70.97%, respectively.The sensitivity and specificity of XpertMTB/RIF in detection of drug resistance were higher than those of TB culture and TB-DNA, and the difference in specificity was statistically significant (χ 2=4.292, 5.415, P=0.038, 0.020). In the fiberoptic bronchoscopy, alveolar lavage and brush biopsy, 75 patients did not have serious adverse reactions.After operation, there were 2 cases with blood in the sputum and 1 case with fever.The adverse reactions of above patients disappeared after 1-3 days. Conclusion:XpertMTB/RIF, TB culture and TB-DNA have good clinical application value in detecting MTB in specimens of patients of tuberculosis, but in terms of rapid detection of tuberculosis and resistance to rifampicin, XpertMTB/RIF detection is more accurate.
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Objective To analyze the clinical curative effect and the impact for denture health by using a fixed denture to repair denti-tion defect of the elderly.Methods A total of 90 elderly patients with dentition defect(118 with tooth)were selected in our hospital from January 2012 to September 2013,who were divided into control group and observation group after the relevant examination,45 cases in each group.The patients in control group were treated by removable dentures,and patients in observation group received fixed denture repair.All patients were followed up for 2 years,the health status and the denture bleeding index between two groups were compared and evaluated by patients feedback and chewing ability tests.Results The clinical total effective rate of observation group after treatment was obviously higher than that of control group,the difference was statistically significant(P <0.05).The health effective rate of abutments of the observation group after 2 years was significantly higher than that of the control group,the difference was statistically significant(P <0.05).Bleeding index of two groups after repair were significantly lower than those before,the difference was statistically significant(P <0.05),which reduced more obviously than control group,the difference was statistically significant(P <0.05).Conclusion The fixed denture has high clinical value for elderly patients with dentition defect,which can effectively repair the complex tooth and tooth defect,with good effect and high safety.
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AIM: To detect the effect of intercellular adhesion molecule-1(ICAM-1) monoclonal antibody on microcirculation disorder in burn shock of Wistar rats. METHODS: The blood flow velocity and diameter of venule were measured with RBC tracking correlator and IV550 model video microscaler in burn shock models of rats. The number of leukocytes adhered on venule wall was calculated under microscope. The animal survival time was observed. RESULTS: ICAM-1monoclonal antibody could attenuate the falling of mean arterial pressure, significantly reduce the number of leukocytes adhered on venule wall, and obviously prolong the animal mean survival time, but less than 24h. CONCLUSION: ICAM-1 monoclonal antibody can decrease the number of adhered leukocytes to endothelial cells, attenuate the tether of leukocytes to venule and improve microcirculation and protect tissue cells in burn shock of rats. However, a comprehensive therapy should be taken in severe shock.